pcDNA3.1-Laconic乳酸传感质粒 货号：P2206
来源:
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作者:shhebio
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发布时间: 2017-12-08
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332 次浏览
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信息
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别名
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乳酸传感
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启动子
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CMV
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复制子
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pUC
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终止子
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SV40 poly(A) signal,bGH poly(A)
signal
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质粒分类
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哺乳细胞，乳酸生物传感器
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质粒大小
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7648bp
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原核抗性
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Amp
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克隆菌株
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DH5α
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培养条件
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37℃
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表达宿主
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哺乳细胞
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诱导方式
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无须诱导，瞬时表达
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5'测序引物
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CMV-F：CGCAAATGGGCGGTAGGCGTG
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3'测序引物
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BGH:TAGAAGGCACAGTCGAGG
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质粒宿主
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哺乳细胞
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质粒用途
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蛋白表达
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片段类型
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基因CDS
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原核抗性
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氨苄青霉素
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简介
Lactate is shuttled between and inside cells, playing metabolic and signaling roles in healthy tissues. Lactate is also a harbinger of altered metabolism and participates in the pathogenesis of inflammation, hypoxia/ischemia, neurodegeneration and cancer. Many tumor cells show high rates of lactate production in the presence of oxygen, a phenomenon known as the Warburg effect, which has diagnostic and possibly therapeutic implications. In this article we introduce Laconic, a genetically-encoded Forster Resonance Energy Transfer (FRET)-based lactate sensor designed on the bacterial transcription factor LldR. Laconic quantified lactate from 1 µM to 10 mM and was not affected by glucose, pyruvate, acetate,
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