pVL1392 and pVL1393 are 9.8 kb vectors designed for use as baculovirus transfer vectors. These transfer vectors contain recombination sequences which are homologous to sequences in the baculovirus genome.Upon cotransfection, these sequences undergo homologous recombination resulting in a recombinant viral genome packaged in infectious viral particles.
    These vectors are nonfusion vectors, which provide the AcMNPV polyhedrin enhancer-promoter sequences to drive high expression, and a multiple cloning site, conveniently constructed in opposite orientations, for simple subcloning. Translation initiation sequences must be provided by the inserted cDNA.
    • Multiple cloning site polylinker, positioned in opposite orientations
    •  Recombination sequences for insertion into the baculovirus genome
    • Polyhedrin enhancer-promoter sequences for high expression of recombinant protein Features for growth and maintenance in E. coli:
    • Ampicillin gene for selection
    • pMB1 origin of replication from pUC19 for high copy number growth