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您现在的位置:
启动子 | ADH1 promoter |
复制子 | pUC ori,F1 ori,2μ ori |
终止子 | CYC1 terminator |
质粒分类 | 酵母细胞质粒;酵母杂交质粒;双杂交类质粒 |
质粒大小 | 6620bp |
质粒标签 | C-HA,C-NubG |
原核抗性 | Amp |
真核抗性 | TRP1 |
克隆菌株 | DH5α |
培养条件 | 37℃ |
表达宿主 | 酵母菌 |
培养条件 | 30℃,YPD,有氧 |
备注 | 高拷贝质粒 |
质粒宿主 | 酵母菌 |
质粒用途 | 杂交 |
原核抗性 | 氨苄青霉素 |
真核抗性 | TRP1 |
pPR3-C是一个用于膜蛋白酵母双杂交实验的质粒。The DUALmembrane system is intended for the detection and identification
of interactions involving integral membrane proteins and
membrane-associated proteins in yeast. The DUALmembrane pairwise
interaction kit enables you to:
Investigate the interaction between a membrane protein (integral
or membrane-associated) and a membrane protein or soluble protein
Map domains or amino acids which are critical for an interaction
Screen cDNA libraries using a membrane protein as a bait to find
novel
interacting proteins (requires purchase of a separate cDNA library
from Dualsystems)
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