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网站首页 >> pCAMBIA1391植物表达质粒 货号：P0625
pCAMBIA1391植物表达质粒 货号：P0625

信息

启动子 无
复制子 pVS1 oriV，pUC ori
终止子 NOS terminator
质粒大小 10658bp
原核抗性 Kan
真核抗性 Hyg
克隆菌株 Stbl3
培养条件 37℃
质粒宿主 植物
质粒用途 蛋白表达
原核抗性 卡那霉素
真核抗性 潮霉素

简介

        pCAMBIA1391是一个植物启动子检测报告载体，可通过MCS克隆进需要研究的启动子。   
       The pCambia vector backbone is derived from the pPZP vectors. The pCambia1391 vector offers:
              High copy number in E.coli for high DNA yields  pVS1 replicon for high stability in Agrobacterium
              Small size  Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest 
              Bacterial selection with kanamycin 
              Plant selection with hygromycin B 
              Simple means to construct translational fusions to gusA reporter genes 
       Plant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV35S promoter and terminated by the CaMV35S polyA signal. Reporter genes feature a hexa-Histidine tag at the C-terminus to enable simple purification on immobilized metal affinity chromatography resins. Designed to utilize gusA as a true reporter of gene expression by fusion construction this vector contains a promoterless, non-intron gusA gene that retains the initiation codon of the NcoI site. This permits simple construction of carboxy-terminus protein fusions to gusA.

图谱


garmin 230